Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.278295
Description:cholinergic receptor, nicotinic, epsilon polypeptide
Best-Of-UniGene (BOU) SequenceNM_000080
Genomic Coordiantes Displayed:Bases 590399 to 597819 of contig Hs17_10980_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.278295.jpeg
Best sense EST/protein match:NM_000080 matched ref|NP_000071.1| (NM_000080) nicotinic acetylcholine receptor epsilon polypeptide (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI983489 cDNA clone IMAGE:2510838 pancreas 3' read 2.4 kbPolyadenylation signal
  F02169 cDNA clone c0ra10 brain 3' read Polyadenylation signal
  AI890843 cDNA clone IMAGE:2442402 uterus 3' read 1.8 kbPolyadenylation signal
  AA336188 cDNA clone ATCC:137876 testis 5' read
  BG236453 cDNA clone IMAGE:4142260 lymphocyte 3' read Protein similarity