Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.301947
Description:kraken-like
Best-Of-UniGene (BOU) SequenceNM_014509
Genomic Coordiantes Displayed:Bases 21924000 to 22106000 of contig Hs22_11677_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.301947.jpeg
Best sense EST/protein match:NM_014509 matched ref|NP_055324.1| (NM_014509) kraken-like [Homo sapiens] (E = e-115)
Best antisense EST/protein match:BI830607 matched ref|XP_013015.2| (XM_013015) CGI-96 protein [Homo sapiens] (E = e-102)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG828721 cDNA clone IMAGE:4905318 rhabdomyosarcoma 5' read
  BG395933 cDNA clone IMAGE:4580984 retinoblastoma 5' read
  BF307799 cDNA clone IMAGE:4131957 rhabdomyosarcoma 5' read
  BI195365 cDNA clone IMAGE:5092571 neuroblastoma 5' read
  T31774 cDNA clone ATCC:102045 whole embryo 5' read
  AI168007 cDNA clone IMAGE:1682461 3' read 0.5 kb
  BI768330 cDNA clone IMAGE:5203327 pooled lung and spleen 5' read Protein similarity
  BI772059 cDNA clone IMAGE:5208750 pooled lung and spleen 5' read Protein similarity
  BI830607 cDNA clone IMAGE:5165408 medulla 5' read Protein similarity
  BI917512 cDNA clone IMAGE:5249062 brain 5' read Protein similarity
  BI915045 cDNA clone IMAGE:5241680 brain 5' read Protein similarity
  BF304711 cDNA clone IMAGE:4122376 rhabdomyosarcoma 5' read Protein similarity
  AL603051 cDNA clone DKFZp686O2320 human skeletal muscle 5' read Protein similarity
  BG390251 cDNA clone IMAGE:4524650 embryonal carcinoma, cell line 5' read Protein similarity
  BI253456 cDNA clone IMAGE:5113077 choriocarcinoma 5' read Protein similarity
  BG395827 cDNA clone IMAGE:4581002 retinoblastoma 5' read Protein similarity
  BG470664 cDNA clone IMAGE:4634135 retinoblastoma 5' read Protein similarity
  BG473261 cDNA clone IMAGE:4646900 retinoblastoma 5' read Protein similarity
  BG540871 cDNA clone IMAGE:4694405 lung 5' read Protein similarity
  BG825249 cDNA clone IMAGE:4900792 rhabdomyosarcoma 5' read Protein similarity
  AL601056 cDNA clone DKFZp313I0739 5' read Protein similarity

SENSE ESTs

  F07091 cDNA clone c1va09 brain
  H55437 cDNA clone C22_491 5' read Protein similarity
  F03366 cDNA clone c1va09 brain 3' read Polyadenylation signal
  R45964 cDNA clone IMAGE:35575 brain 3' read 1.5 kbPolyadenylation signal