Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.325978
Description:interleukin 18 binding protein
Best-Of-UniGene (BOU) SequenceNM_005699
Genomic Coordiantes Displayed:Bases 3190000 to 3260000 of contig Hs11_9453_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.325978.jpeg
Best sense EST/protein match:NM_005699 matched ref|NP_005690.1| (NM_005699) interleukin 18 binding protein [Homo sapiens] (E = e-104)
Best antisense EST/protein match:BG754891 matched pir||S33413 NuMA protein - human (E = 1e-22)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG754891 cDNA clone IMAGE:4854476 primary b-cells from tonsils (cell line) 5' read Protein similarity
  BF338770 cDNA clone IMAGE:4184226 glioblastoma with egfr amplification 5' read
  AA679293 cDNA clone IMAGE:432093 pool 3' read Polyadenylation signal
  BG152573 cDNA clone IMAGE:4147935 glioblastoma without egfr amplification 3' read Polyadenylation signal
  AI358816 cDNA clone IMAGE:2001376 lymph 3' read 1.5 kbPolyadenylation signal
  AW118236 cDNA clone IMAGE:2606869 uterus 3' read Polyadenylation signal
  F17278 cDNA clone NOTAVAIL01806
  BE328787 cDNA clone IMAGE:3145787 2 pooled wilms' tumors, one primary and one metastatic to 3' read Polyadenylation signal
  AI800178 cDNA clone IMAGE:2219172 ovary 3' read Polyadenylation signal
  AI672196 cDNA clone IMAGE:2288210 uterus 3' read Polyadenylation signal
  AI633172 cDNA clone IMAGE:2287870 uterus 3' read 1.7 kbPolyadenylation signal
  AI476472 cDNA clone IMAGE:2156800 colon 3' read 1.1 kbPolyadenylation signal
  AI800167 cDNA clone IMAGE:2219148 ovary 3' read Polyadenylation signal
  AI446547 cDNA clone IMAGE:2140609 stomach 3' read 1.0 kbPolyadenylation signal
  AI275920 cDNA clone IMAGE:1990168 uterus 3' read 0.6 kbPolyadenylation signal
  AA912374 cDNA clone IMAGE:1537659 peripheral nervous system 3' read 2.2 kbPolyadenylation signal
  AI734844 cDNA clone IMAGE:2335863 colon 3' read Polyadenylation signal
  W90116 cDNA clone IMAGE:418129 pool 3' read Polyadenylation signal
  AI913078 cDNA clone IMAGE:2294459 pancreas 3' read 2.9 kbPolyadenylation signal
  AI982885 cDNA clone IMAGE:2559468 three pooled meningiomas 3' read 1.5 kbPolyadenylation signal
  AI811034 cDNA clone IMAGE:2217199 ovary 3' read Polyadenylation signal
  F03666 cDNA clone c25f11 brain 3' read Polyadenylation signal
  AI720515 cDNA clone IMAGE:2335071 colon 3' read Polyadenylation signal
  AI709010 cDNA clone IMAGE:2332344 colon 3' read Polyadenylation signal
  AI801564 cDNA clone IMAGE:2185628 stomach 3' read 1.4 kbPolyadenylation signal
  T67737 cDNA clone IMAGE:81938 liver 3' read 0.8 kbPolyadenylation signal
  F10588 cDNA clone c3hc06 brain 3' read Polyadenylation signal
  BF239926 cDNA clone IMAGE:4132925 from chronic myelogenous leukemia 5' read Protein similarity
  AA336221 cDNA clone ATCC:137910 testis 5' read Protein similarity
  AA335942 cDNA clone ATCC:137621 testis 5' read Protein similarity
  AA363836 cDNA clone ATCC:168539 brain 5' read Protein similarity

SENSE ESTs

  BI824811 cDNA clone IMAGE:5174835 pooled brain, lung, testis 5' read Protein similarity
  BI521608 cDNA clone IMAGE:5220396 pooled pancreas and spleen 5' read Protein similarity
  BI824342 cDNA clone IMAGE:5181830 pooled brain, lung, testis 5' read Protein similarity
  BG696792 cDNA clone IMAGE:4803248 skin 5' read
  AA297617 cDNA clone ATCC:146539 gall bladder 5' read Protein similarity
  AA297872 cDNA clone ATCC:154478 5' read Protein similarity
  BI822872 cDNA clone IMAGE:5181242 pooled brain, lung, testis 5' read Protein similarity
  BG775971 cDNA clone IMAGE:4761073 carcinoma, cell line 5' read Protein similarity
  AA302972 cDNA clone ATCC:145044 gall bladder 5' read Protein similarity
  BI829728 cDNA clone IMAGE:5171399 medulla 5' read Protein similarity
  AA311795 cDNA clone ATCC:159026 5' read Protein similarity
  BF724662 cDNA clone bx07d09 iris 5' read Protein similarity
  AI674844 cDNA clone IMAGE:2324646 pancreas 3' read 1.4 kb
  AI521549 cDNA clone IMAGE:2183078 stomach 3' read 1.3 kb
  AA777410 cDNA clone IMAGE:449043 pool 3' read
  AW519013 cDNA clone IMAGE:2876923 pancreas 3' read
  AI889935 cDNA clone IMAGE:2440816 uterus 3' read 1.7 kb
  AI473445 cDNA clone IMAGE:2141720 stomach 3' read 1.5 kb
  T28615 cDNA clone ATCC:103014 gall bladder 5' read