Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.326248
Description:Homo sapiens mRNA; cDNA DKFZp564H2416 (from clone DKFZp564H2416)
Best-Of-UniGene (BOU) SequenceAK025724
Genomic Coordiantes Displayed:Bases 473000 to 522000 of contig Hs10_8826_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.326248.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:AU138881 matched pir||JC5193 nuclear protein H731 - human (E = 3e-29)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW172905 cDNA clone IMAGE:2656354 uterus 3' read Polyadenylation signal
  AI225002 cDNA clone IMAGE:1998813 stomach 3' read Polyadenylation signal
  AI814004 cDNA clone IMAGE:2421765 pancreas 3' read 1.2 kbPolyadenylation signal
  AI884575 cDNA clone IMAGE:2437856 uterus 3' read 2.0 kbPolyadenylation signal
  AW192359 cDNA clone IMAGE:2677562 pancreas 3' read Polyadenylation signal
  AW150173 cDNA clone IMAGE:2629654 uterus 3' read Polyadenylation signal
  AA908461 cDNA clone IMAGE:1454712 ovary 3' read 0.5 kbPolyadenylation signal
  AA679599 cDNA clone IMAGE:1128478 kidney 3' read Polyadenylation signal
  AW188720 cDNA clone IMAGE:2673687 uterus 3' read Polyadenylation signal
  AW058311 cDNA clone IMAGE:2544050 stomach 3' read Polyadenylation signal
  BI224250 cDNA clone IMAGE:5103634 choriocarcinoma 5' read
  AI683803 cDNA clone IMAGE:2275596 uterus 3' read 0.8 kbPolyadenylation signal
  BF975313 cDNA clone IMAGE:4337118 primary b-cells from tonsils (cell line) 5' read Polyadenylation signal
  AW173611 cDNA clone IMAGE:2656760 uterus 3' read
  AI866048 cDNA clone IMAGE:2425902 uterus 3' read 0.5 kbPolyadenylation signal
  BG682380 cDNA clone IMAGE:4754930 squamous cell carcinoma 5' read
  AI275793 cDNA clone IMAGE:1990072 uterus 3' read 0.9 kbPolyadenylation signal
  AI925803 cDNA clone IMAGE:2457379 stomach 3' read 3.6 kb
  AI925812 cDNA clone IMAGE:2458950 stomach 3' read 0.4 kb
  AU146855 cDNA clone HEMBB1001692 whole embryo, mainly body 3' read
  AU144100 cDNA clone HEMBA1000881 whole embryo, mainly head 3' read
  AU157798 cDNA clone PLACE1009494 placenta 3' read
  AU156055 cDNA clone PLACE1002094 placenta 3' read
  AU155628 cDNA clone PLACE1000462 placenta 3' read
  BG741428 cDNA clone IMAGE:4777251 skin 5' read Protein similarity
  AA090734 cDNA clone (no-name) heart 5' read
  BG696321 cDNA clone IMAGE:4802586 skin 5' read
  BG616647 cDNA clone IMAGE:4733529 liver 5' read
  AI343562 cDNA clone IMAGE:1917657 kidney 3' read 0.7 kbPolyadenylation signal
  AW196927 cDNA clone IMAGE:2576216 2 pooled wilms' tumors, one primary and one metastatic to 3' read Polyadenylation signal
  AI696935 cDNA clone IMAGE:2324519 pancreas 3' read Polyadenylation signal
  T24011 cDNA clone 3HFLSK2053 pool 3' read Polyadenylation signal
  AW071510 cDNA clone IMAGE:2569695 five pooled sarcomas, including myxoid liposarcoma, solitary 3' read Polyadenylation signal
  AI866025 cDNA clone IMAGE:2425833 uterus 3' read 0.7 kbPolyadenylation signal
  BF970558 cDNA clone IMAGE:4362012 adrenal cortex carcinoma, cell line 5' read
  BG528581 cDNA clone IMAGE:4719093 adenocarcinoma 5' read
  BF571668 cDNA clone IMAGE:4243708 melanotic melanoma, high mdr 5' read
  BF184075 cDNA clone IMAGE:4063897 from chronic myelogenous leukemia 5' read
  AW512785 cDNA clone IMAGE:2792500 uterus 3' read Polyadenylation signal
  AA873455 cDNA clone IMAGE:1472318 kidney 3' read 1.4 kbPolyadenylation signal
  AI872815 cDNA clone IMAGE:2441390 uterus 3' read Polyadenylation signal
  AW087937 cDNA clone IMAGE:2578817 lung, cell line 3' read Polyadenylation signal
  AI832283 cDNA clone IMAGE:2377030 colon 3' read Polyadenylation signal
  AU156150 cDNA clone PLACE1002428 placenta 3' read Polyadenylation signal
  AU156657 cDNA clone PLACE1004040 placenta 3' read Polyadenylation signal
  AU157459 cDNA clone PLACE1007817 placenta 3' read Polyadenylation signal
  AW467298 cDNA clone IMAGE:2918464 myeloid cells, 18 pooled cml cases, bcr/abl rearrangement 3' read Polyadenylation signal
  AW192938 cDNA clone IMAGE:2678644 pancreas 3' read Polyadenylation signal
  AU156691 cDNA clone PLACE1004126 placenta 3' read Polyadenylation signal
  D61811 cDNA clone GEN212H08 aorta 5' read
  AU138881 cDNA clone PLACE1009494 placenta 5' read Protein similarity
  D78737 cDNA clone GEN508H11 placenta 5' read
  AU117202 cDNA clone HEMBA1000881 whole embryo, mainly head 5' read Protein similarity
  AU158047 cDNA clone PLACE1010718 placenta 3' read
  AL600748 cDNA clone DKFZp313A0238 5' read Protein similarity