Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.334475
Description:hypothetical protein FLJ10634
Best-Of-UniGene (BOU) SequenceNM_018163
Genomic Coordiantes Displayed:Bases 128000 to 212000 of contig Hs15_10351_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.334475.jpeg
Best sense EST/protein match:NM_018163 matched ref|NP_060633.1| (NM_018163) hypothetical protein FLJ10634 [Homo sapiens] (E = e-115)
Best antisense EST/protein match:BE736164 matched ref|NP_116239.1| (NM_032850) hypothetical protein FLJ14840 [Homo sapiens] (E = 5e-49)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BE736164 cDNA clone IMAGE:3641437 adenocarcinoma 5' read
  BG257984 cDNA clone IMAGE:4510091 embryonal carcinoma, cell line 5' read
  BG753662 cDNA clone IMAGE:4876596 normal pigmented retinal epithelium 5' read
  BG339812 cDNA clone IMAGE:4555995 leiomyosarcoma cell line 5' read
  BG707269 cDNA clone IMAGE:4795428 hypothalamus, cell line 5' read
  BI914670 cDNA clone IMAGE:5243633 brain 5' read

SENSE ESTs

  BE314717 cDNA clone IMAGE:3507470 neuroblastoma 5' read Protein similarity
  BF312404 cDNA clone IMAGE:4128106 neuroblastoma 5' read Protein similarity
  AU129492 cDNA clone NT2RP2005654 5' read Protein similarity