Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.351458
Description:Homo sapiens cDNA FLJ30792 fis, clone FEBRA2001004, highly similar to Human (clone N5-4) protein p84 mRNA
Best-Of-UniGene (BOU) SequenceAK055354
Genomic Coordiantes Displayed:Bases 1914880 to 1962141 of contig Hs18_11162_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.351458.jpeg
Best sense EST/protein match:AK055354 matched ref|XP_008756.4| (XM_008756) nuclear matrix protein p84 [Homo sapiens] (E = e-161)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA583447 cDNA clone IMAGE:1086144 germ cell 3' read 1.2 kbPolyadenylation signal
  BI493357 cDNA clone IMAGE:2540501 ear 5' read Polyadenylation signal
  BI493356 cDNA clone IMAGE:2540501 ear 3' read Polyadenylation signal
  AI536829 cDNA clone IMAGE:2178942 uterus 3' read 1.3 kbPolyadenylation signal
  AW674514 cDNA clone IMAGE:2984940 placenta 3' read Polyadenylation signal
  AW303946 cDNA clone IMAGE:2801317 colon 3' read Polyadenylation signal
  R60591 cDNA clone IMAGE:37964 brain 3' read Polyadenylation signal
  R40348 cDNA clone IMAGE:28909 brain 3' read 1.6 kbPolyadenylation signal

SENSE ESTs

  AL038081 cDNA clone DKFZp566K171 kidney 5' read
  AU119219 cDNA clone HEMBA1005302 whole embryo, mainly head 5' read Protein similarity
  AI783925 cDNA clone IMAGE:2219839 ovary 3' read Protein similarity
  AW089863 cDNA clone IMAGE:2594501 ovary 3' read Protein similarity
  AL602406 cDNA clone DKFZp686G0713 human skeletal muscle 5' read