Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.44104
Description:Homo sapiens cDNA FLJ31377 fis, clone NESOP1000087
Best-Of-UniGene (BOU) SequenceAK055939
Genomic Coordiantes Displayed:Bases 350030 to 414762 of contig Hs5_6612_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.44104.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:BI549984 matched ref|NP_071413.1| (NM_022130) golgi phosphoprotein 3 (coat-protein); Golgi protein; (E = 2e-23)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI092694 cDNA clone IMAGE:4999629 placenta 5' read
  BI333915 cDNA clone IMAGE:5141486 choriocarcinoma 5' read Protein similarity
  BG176629 cDNA clone IMAGE:4419053 lymphoma, cell line 5' read Protein similarity
  BG180809 cDNA clone IMAGE:4428707 adenocarcinoma, cell line 5' read Protein similarity
  BI549984 cDNA clone IMAGE:5264097 hippocampus, cell line 5' read Protein similarity
  BG824753 cDNA clone IMAGE:4867882 adenocarcinoma cell line 5' read
  BE748129 cDNA clone IMAGE:3838581 from acute myelogenous leukemia 5' read
  BI550451 cDNA clone IMAGE:5264286 hippocampus, cell line 5' read Protein similarity

SENSE ESTs

  AL037070 cDNA clone DKFZp564K1164 brain 3' read
  AL037069 cDNA clone DKFZp564K1164 brain 5' read
  BG435863 cDNA clone IMAGE:4605932 placenta 5' read
  BG165751 cDNA clone IMAGE:4454353 hypernephroma, cell line 5' read
  BG339704 cDNA clone IMAGE:4555029 leiomyosarcoma cell line 5' read
  BI861548 cDNA clone IMAGE:5399497 mammary adenocarcinoma, cell line 5' read