Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.65029
Description:growth arrest-specific 1
Best-Of-UniGene (BOU) SequenceNM_002048
Genomic Coordiantes Displayed:Bases 693000 to 707000 of contig Hs9_24091_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.65029.jpeg
Best sense EST/protein match:NM_002048 matched ref|NP_002039.1| (NM_002048) growth arrest-specific 1; Growth arrest-specific gene-1 (E = e-119)
Best antisense EST/protein match:AI087284 matched ref|XP_072546.1| (XM_072546) hypothetical protein XP_072546 [Homo sapiens] (E = 7e-56)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI086788 cDNA clone IMAGE:1681356 3' read 0.7 kb
  AI087284 cDNA clone IMAGE:1681387 3' read 0.8 kb
  AW263284 cDNA clone IMAGE:2757604 three pooled meningiomas 3' read Protein similarity
  AW516303 cDNA clone IMAGE:2791236 uterus 3' read Protein similarity
  AW167931 cDNA clone IMAGE:2632424 uterus 3' read
  AV729138 cDNA clone HTCBJF05 hypothalamus 5' read

SENSE ESTs

  BG829292 cDNA clone IMAGE:4905862 rhabdomyosarcoma 5' read Protein similarity
  AA371412 cDNA clone ATCC:175832 prostate 5' read
  BF240148 cDNA clone IMAGE:4133485 from chronic myelogenous leukemia 5' read
  AA248414 cDNA clone (no-name) heart 5' read
  W85983 cDNA clone IMAGE:416122 pool 5' read 1.3 kb
  AA248340 cDNA clone (no-name) heart 5' read
  BG676271 cDNA clone IMAGE:4747960 squamous cell carcinoma 5' read
  BG435906 cDNA clone IMAGE:4605231 placenta 5' read
  BE300263 cDNA clone IMAGE:2960380 rhabdomyosarcoma 3' read
  BE619520 cDNA clone IMAGE:3876138 large cell carcinoma 5' read
  BE619835 cDNA clone IMAGE:3876138 large cell carcinoma 3' read Polyadenylation signal
  BF695784 cDNA clone IMAGE:4076191 primitive neuroectoderm 5' read
  BG400405 cDNA clone IMAGE:4592526 kidney 5' read
  BF695673 cDNA clone IMAGE:4076113 primitive neuroectoderm 5' read