Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.66295
Description:multi-PDZ-domain-containing protein
Best-Of-UniGene (BOU) SequenceNM_032622
Genomic Coordiantes Displayed:Bases 348000 to 362000 of contig Hs4_22987_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.66295.jpeg
Best sense EST/protein match:BE535283 matched ref|XP_030360.1| (XM_030360) multi-PDZ-domain-containing protein [Homo sapiens] (E = e-101)
Best antisense EST/protein match:AI963004 matched gb|AAF28989.1|AF161429_1 (AF161429) HSPC311 [Homo sapiens] (E = 2e-46)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI963004 cDNA clone IMAGE:2508556 uterus 3' read 2.1 kbPolyadenylation signal
  AL038906 cDNA clone DKFZp566J0147 kidney 5' read Polyadenylation signal
  BF718630 cDNA clone S90891.NIH2-R epidermis 3' read Polyadenylation signal
  AI690662 cDNA clone IMAGE:2269248 uterus 3' read 2.2 kbPolyadenylation signal
  BG530280 cDNA clone IMAGE:4697041 embryonal carcinoma 5' read
  AV741954 cDNA clone CBLABF11 cord blood 5' read Polyadenylation signal
  AA295614 cDNA clone ATCC:191054 pancreas 5' read
  BF382287 cDNA clone IMAGE:4049160 primitive neuroectoderm 5' read
  BG492831 cDNA clone IMAGE:4655773 mucoepidermoid carcinoma 5' read
  AW272315 cDNA clone IMAGE:2800470 colon 3' read
  BG222374 cDNA clone IMAGE:4169981 glioblastoma with probably tp53 mutation and without egfr 3' read Protein similarityPolyadenylation signal
  Z43018 cDNA clone c10h03 brain Protein similarity
  BG164039 cDNA clone IMAGE:4449156 hypernephroma, cell line 5' read Protein similarity
  BF790298 cDNA clone IMAGE:4327639 muscle (skeletal) 5' read
  D61519 cDNA clone GEN411H04 brain 5' read Protein similarity
  BF941295 cDNA clone IMAGE:3280990 dorsal root ganglia 3' read Protein similarity
  BG573242 cDNA clone IMAGE:4724396 placenta 5' read Protein similarity
  AI709326 cDNA clone IMAGE:2353929 aorta 3' read Polyadenylation signal
  BG612604 cDNA clone IMAGE:4771182 embryonal carcinoma 5' read
  BG171413 cDNA clone IMAGE:4425290 hypernephroma, cell line 5' read
  AI581043 cDNA clone IMAGE:2139924 stomach 3' read 1.0 kb
  BG495626 cDNA clone IMAGE:4671063 mucoepidermoid carcinoma 5' read
  Z39122 cDNA clone c10h03 brain 3' read

SENSE ESTs

  Z38222 cDNA clone c03c12 brain 3' read
  F03153 cDNA clone c1nf01 brain 3' read
  T35290 cDNA clone ATCC:106511 prostate 3' read
  F03307 cDNA clone c1sh02 brain 3' read Polyadenylation signal
  Z39956 cDNA clone c1nc12 brain 3' read
  R40351 cDNA clone IMAGE:28914 brain 3' read 1.6 kbPolyadenylation signal
  AI499299 cDNA clone IMAGE:2178596 uterus 3' read 2.9 kbPolyadenylation signal
  R49735 cDNA clone IMAGE:38558 brain 3' read Polyadenylation signal
  T35291 cDNA clone ATCC:106511 prostate 5' read Protein similarity
  T35286 cDNA clone ATCC:106502 prostate 5' read Protein similarity
  BI825467 cDNA clone IMAGE:5164034 medulla 5' read Protein similarity
  F05185 cDNA clone c03c12 brain Protein similarity
  BE535283 cDNA clone IMAGE:3445492 placenta 5' read Protein similarity
  R49734 cDNA clone IMAGE:38558 brain 5' read Protein similarity
  Z44116 cDNA clone c1sh02 brain Protein similarity
  BG714901 cDNA clone IMAGE:4798332 hypothalamus, cell line 5' read Protein similarity
  BI758666 cDNA clone IMAGE:5194423 brain 5' read Protein similarity
  BI756443 cDNA clone IMAGE:5199278 brain 5' read Protein similarity
  BI760157 cDNA clone IMAGE:5184880 pooled colon, kidney, stomach 5' read Protein similarity
  BI090632 cDNA clone IMAGE:4995278 placenta 5' read Protein similarity
  BI601489 cDNA clone IMAGE:5300803 hypothalamus, cell line 5' read Protein similarity
  Z43893 cDNA clone c1nc12 brain Protein similarity
  F05619 cDNA clone c0ha02 brain Protein similarity
  F06885 cDNA clone c1nf01 brain Protein similarity