Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.9061
Description:hypothetical protein MGC2477
Best-Of-UniGene (BOU) SequenceNM_024099
Genomic Coordiantes Displayed:Bases 936000 to 964000 of contig Hs11_9471_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.9061.jpeg
Best sense EST/protein match:AI034371 matched ref|NP_077004.1| (NM_024099) hypothetical protein MGC2477 [Homo sapiens] (E = 2e-43)
Best antisense EST/protein match:AI440222 matched ref|XP_071722.1| (XM_071722) hypothetical protein XP_071722 [Homo sapiens] (E = 2e-47)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BM126802 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  BE973782 cDNA clone IMAGE:3951163 prostate 5' read
  BE906085 cDNA clone IMAGE:3899309 epithelioid carcinoma 5' read
  BM127114 cDNA clone (no-name) islets of langerhans 5' read Polyadenylation signal
  BF668668 cDNA clone IMAGE:4280622 primitive neuroectoderm 5' read
  BG385605 cDNA clone IMAGE:4582393 adenocarcinoma cell line 5' read Polyadenylation signal
  BG034818 cDNA clone IMAGE:4413376 adenocarcinoma, cell line 5' read Protein similarity
  BE615507 cDNA clone IMAGE:3610949 adenocarcinoma 5' read
  BI520531 cDNA clone IMAGE:5163538 medulla 5' read
  BE909060 cDNA clone IMAGE:3901330 epithelioid carcinoma 5' read
  BI966894 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  BI963097 cDNA clone (no-name) islets of langerhans 5' read Polyadenylation signal
  BG180507 cDNA clone IMAGE:4432566 adenocarcinoma, cell line 5' read
  AI698464 cDNA clone IMAGE:2274473 uterus 3' read 0.7 kbPolyadenylation signal
  AA954006 cDNA clone IMAGE:1573513 kidney 3' read 0.6 kbPolyadenylation signal
  AA876361 cDNA clone IMAGE:1493094 kidney 3' read 0.7 kbPolyadenylation signal
  AI440222 cDNA clone IMAGE:2140188 stomach 3' read 0.7 kbPolyadenylation signal
  F32394 cDNA clone s3000039D12 muscle
  F37224 cDNA clone sH5000025-0/A12 muscle
  F33504 cDNA clone s3000061D12 muscle
  AA582136 cDNA clone IMAGE:1085993 germ cell 3' read 0.6 kb
  AA056404 cDNA clone IMAGE:509531 3' read 0.7 kb
  AA056530 cDNA clone IMAGE:509531 5' read 0.7 kb

SENSE ESTs

  AL039917 cDNA clone DKFZp434H1812 testis 5' read
  BG472416 cDNA clone IMAGE:4645892 retinoblastoma 5' read
  BG473341 cDNA clone IMAGE:4647257 retinoblastoma 5' read
  BG328968 cDNA clone IMAGE:4558588 retinoblastoma 5' read
  BE258807 cDNA clone IMAGE:3357941 retinoblastoma 5' read
  BE255555 cDNA clone IMAGE:3352333 retinoblastoma 5' read
  BE252507 cDNA clone IMAGE:3349691 retinoblastoma 5' read
  AA311811 cDNA clone ATCC:159242 5' read
  BG336304 cDNA clone IMAGE:4542965 choriocarcinoma 5' read
  BG336391 cDNA clone IMAGE:4542966 choriocarcinoma 5' read
  AA121105 cDNA clone IMAGE:526405 5' read
  BE280823 cDNA clone IMAGE:3138904 choriocarcinoma 5' read
  BE622405 cDNA clone IMAGE:3916223 melanotic melanoma 5' read
  BG681808 cDNA clone IMAGE:4754360 squamous cell carcinoma 5' read
  AA378918 cDNA clone ATCC:183323 synovial membrane 5' read
  BE967121 cDNA clone IMAGE:3916223 melanotic melanoma 3' read Polyadenylation signal
  BI550350 cDNA clone IMAGE:5276013 hippocampus, cell line 5' read
  BI552960 cDNA clone IMAGE:5277549 hippocampus, cell line 5' read
  AA327056 cDNA clone ATCC:127716 colon 5' read
  BG563566 cDNA clone IMAGE:4709805 liver 5' read
  BF207356 cDNA clone IMAGE:4100923 neuroblastoma 5' read
  BG165776 cDNA clone IMAGE:4454457 hypernephroma, cell line 5' read
  AA007358 cDNA clone IMAGE:429287 pool 5' read
  AV736666 cDNA clone CBNACA03 cord blood 5' read
  AA114213 cDNA clone IMAGE:564015 5' read 0.4 kb
  BG823933 cDNA clone IMAGE:4866892 adenocarcinoma cell line 5' read
  BE382582 cDNA clone IMAGE:3628628 neuroblastoma 5' read
  AA328677 cDNA clone ATCC:130328 whole embryo 5' read
  BI831485 cDNA clone IMAGE:5166503 medulla 5' read
  BE897408 cDNA clone IMAGE:3924098 melanotic melanoma 5' read
  AA306433 cDNA clone ATCC:116540 aorta 5' read
  BI547155 cDNA clone IMAGE:5261735 hippocampus, cell line 5' read
  BF028466 cDNA clone IMAGE:3995992 carcinoma, cell line 5' read
  BG481058 cDNA clone IMAGE:4652769 choriocarcinoma 5' read
  AA313961 cDNA clone ATCC:110526 5' read
  BG681685 cDNA clone IMAGE:4752696 squamous cell carcinoma 5' read
  BG752339 cDNA clone IMAGE:4874570 normal pigmented retinal epithelium 5' read
  BE789037 cDNA clone IMAGE:3883314 large cell carcinoma 5' read
  AA302620 cDNA clone ATCC:114797 aorta 5' read
  BG288112 cDNA clone IMAGE:4516562 transitional cell papilloma, cell line 5' read Polyadenylation signal
  AW958015 cDNA clone (no-name)
  AA314008 cDNA clone ATCC:110541 5' read
  BE385463 cDNA clone IMAGE:3616957 melanotic melanoma 5' read
  BE746244 cDNA clone IMAGE:3928374 adenocarcinoma cell line 5' read
  BE743768 cDNA clone IMAGE:3926682 adenocarcinoma cell line 5' read
  BE746431 cDNA clone IMAGE:3928359 adenocarcinoma cell line 5' read
  T49801 cDNA clone IMAGE:68636 placenta 5' read 0.6 kb
  AA311453 cDNA clone ATCC:157400 5' read
  AI034371 cDNA clone IMAGE:1656914 pool 3' read 0.7 kb
  AA526380 cDNA clone IMAGE:984619 prostate 3' read Polyadenylation signal
  AV688530 cDNA clone GKCDFE04 hepatocellular carcinoma 5' read
  AA658053 cDNA clone IMAGE:1174618 prostate 3' read 0.4 kbPolyadenylation signal
  BE617845 cDNA clone IMAGE:3846053 adenocarcinoma 3' read
  AW103096 cDNA clone IMAGE:2596533 ovary 3' read Polyadenylation signal
  AI859686 cDNA clone IMAGE:2436384 uterus 3' read 0.7 kbPolyadenylation signal
  BG575294 cDNA clone IMAGE:4706867 mammary adenocarcinoma, cell line 5' read Polyadenylation signal