Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.94694
Description:Homo sapiens cDNA FLJ10561 fis, clone NT2RP2002672
Best-Of-UniGene (BOU) SequenceAK056108
Genomic Coordiantes Displayed:Bases 489904 to 495955 of contig Hs10_9056_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.94694.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  R40611 cDNA clone IMAGE:27931 brain 3' read 1.0 kb
  H05316 cDNA clone IMAGE:44338 brain 3' read 1.2 kb
  Z38769 cDNA clone c0of01 brain 3' read
  R15226 cDNA clone IMAGE:29757 brain 5' read 1.0 kb
  H05370 cDNA clone IMAGE:44338 brain 5' read 1.2 kb
  R13132 cDNA clone IMAGE:27931 brain 5' read 1.0 kb
  Z42589 cDNA clone c0of01 brain
  R05662 cDNA clone IMAGE:29502 brain 5' read 1.1 kb

SENSE ESTs

  BG026710 cDNA clone IMAGE:4388294 osteosarcoma, cell line 5' read