Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.18210
Description:mutS homolog 6 (E. coli)
Best-Of-UniGene (BOU) SequenceNM_010830
Genomic Coordiantes Displayed:Bases 5153527 to 5200280 of contig GA_x5J8B7W7FUK
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.18210.jpeg
Best sense EST/protein match:AA117010 matched ref|NP_034960.1| (NM_010830) mutS homolog 6 (E. coli) [Mus musculus] (E = e-108)
Best antisense EST/protein match:BE288168 matched gb|AAH12728.1|AAH12728 (BC012728) Unknown (protein for MGC:14931) [Homo sapiens] (E = e-102)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF116663 cDNA clone IMAGE:3666889 tumor, gross tissue 5' read Protein similarity
  BI665100 cDNA clone IMAGE:5321396 infiltrating ductal carcinoma 5' read Protein similarity
  BF540331 cDNA clone IMAGE:4191572 salivary gland 5' read Protein similarity
  BG800639 cDNA clone (no-name) neural retina
  BE633109 cDNA clone IMAGE:3412777 spleen 5' read Protein similarity
  BF022282 cDNA clone IMAGE:3663459 tumor, metastatic to mammary 5' read Protein similarity
  AA104938 cDNA clone IMAGE:533479 macrophage 5' read
  BE288168 cDNA clone IMAGE:3489951 tumor, gross tissue 5' read Protein similarity
  AA920054 cDNA clone IMAGE:1311312 macrophage 5' read
  W91720 cDNA clone MTA.H08.077 thymus 5' read
  BF151017 cDNA clone IMAGE:3666889 tumor, gross tissue 3' read
  AL364420 cDNA clone B9C20C58 embryo 5' read
  AA437748 cDNA clone IMAGE:805794 embryo 5' read
  AA590387 cDNA clone IMAGE:990322 embryo 5' read
  BE533335 cDNA clone IMAGE:3598147 infiltrating ductal carcinoma 5' read
  C80471 cDNA clone J0082F04 embryo 3' read
  AA199271 cDNA clone IMAGE:657916 embryo 5' read
  BG081793 cDNA clone H3069C02 5' read
  BI452664 cDNA clone IMAGE:5249441 tumor, gross tissue 5' read
  AA529060 cDNA clone IMAGE:905460 colon 5' read
  BE336071 cDNA clone IMAGE:3325035 spleen 5' read
  AU023779 cDNA clone J0438F11 unfertilized egg 3' read Polyadenylation signal
  BF319228 cDNA clone IMAGE:3663459 tumor, metastatic to mammary 3' read Polyadenylation signal
  BE686104 cDNA clone IMAGE:3412777 spleen 3' read Polyadenylation signal
  AU017787 cDNA clone J0744D01 embryo 3' read Polyadenylation signal
  BG068779 cDNA clone H3069C02 3' read

SENSE ESTs

  AA536842 cDNA clone IMAGE:934649 embryo 5' read Protein similarity
  C86635 cDNA clone J0230C04 embryo 3' read Protein similarityPolyadenylation signal
  AU046157 cDNA clone J0947F04 3' read Protein similarityPolyadenylation signal
  BG073552 cDNA clone H3124A04 3' read Protein similarityPolyadenylation signal
  AA245802 cDNA clone IMAGE:679010 liver 5' read Protein similarity
  AU045734 cDNA clone J0940E06 3' read Protein similarityPolyadenylation signal
  AA529407 cDNA clone IMAGE:905840 embryo 5' read Protein similarity
  AU046053 cDNA clone J0945H10 3' read Protein similarityPolyadenylation signal
  BM053406 cDNA clone (no-name) 3' read Polyadenylation signal
  AA560919 cDNA clone IMAGE:977563 embryo 5' read Protein similarity
  C86385 cDNA clone J0225E10 embryo 3' read Protein similarityPolyadenylation signal
  BE570846 cDNA clone IMAGE:3603230 infiltrating ductal carcinoma 5' read Protein similarity
  BG060409 cDNA clone L0902F04 3' read Protein similarityPolyadenylation signal
  BI691233 cDNA clone IMAGE:5346921 infiltrating ductal carcinoma 5' read Protein similarity
  AI324995 cDNA clone IMAGE:541252 embryo 3' read Protein similarityPolyadenylation signal
  BI793459 cDNA clone (no-name) 3' read Protein similarityPolyadenylation signal
  AW822490 cDNA clone IMAGE:2803160 thymus 3' read Protein similarityPolyadenylation signal
  BE225641 cDNA clone (no-name) pancreatic bud 3' read Protein similarityPolyadenylation signal
  AU046031 cDNA clone J0945E10 3' read Protein similarityPolyadenylation signal
  BG086280 cDNA clone H3124A04 5' read Protein similarity
  BF142505 cDNA clone IMAGE:4016255 tumor, metastatic to mammary 5' read Protein similarity
  BI665092 cDNA clone IMAGE:5321632 infiltrating ductal carcinoma 5' read Protein similarity
  AA895038 cDNA clone IMAGE:1280116 macrophage 5' read Protein similarity
  BI738067 cDNA clone IMAGE:5364614 retina 5' read Protein similarity
  BI792713 cDNA clone (no-name) 5' read Protein similarity
  BF321102 cDNA clone IMAGE:3673428 infiltrating ductal carcinoma 5' read Protein similarity
  AI324481 cDNA clone IMAGE:541252 embryo 5' read Protein similarity
  AA563252 cDNA clone IMAGE:977990 embryo 5' read Protein similarity
  D76910 cDNA clone 70A11 embryonal carcinoma Protein similarity
  AA162844 cDNA clone IMAGE:541252 embryo 5' read Protein similarity
  BG173101 cDNA clone IMAGE:4460008 tumor, biopsy sample 5' read Protein similarity
  AA117010 cDNA clone IMAGE:538642 embryo 5' read Protein similarity
  BI987600 cDNA clone (no-name) neural retina
  BG920369 cDNA clone IMAGE:4954923 infiltrating ductal carcinoma 5' read Protein similarity
  BM053722 cDNA clone (no-name) 5' read
  BG061109 cDNA clone L0902F04 5' read Protein similarity
  BG172975 cDNA clone IMAGE:4459760 tumor, biopsy sample 5' read Protein similarity
  AW822905 cDNA clone IMAGE:2803160 thymus 5' read Protein similarity
  BE290726 cDNA clone IMAGE:3498039 infiltrating ductal carcinoma 5' read Protein similarity
  BG519310 cDNA clone IMAGE:3498039 infiltrating ductal carcinoma 5' read Protein similarity
  BI558639 cDNA clone IMAGE:5293595 tumor, gross tissue 5' read Protein similarity
  BI081393 cDNA clone IMAGE:5011024 tumor, biopsy sample 5' read Protein similarity
  BG923862 cDNA clone IMAGE:4953238 infiltrating ductal carcinoma 5' read Protein similarity