Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.183060
Description:RIKEN cDNA 2310076G09 gene
Best-Of-UniGene (BOU) SequenceAK010198
Genomic Coordiantes Displayed:Bases 57892 to 62405 of contig GA_x5J8B7W33JC
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.183060.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW209158 cDNA clone IMAGE:2582243 infiltrating ductal carcinoma 3' read Polyadenylation signal
  AW536262 cDNA clone G0102D07 3' read Polyadenylation signal
  BG064858 cDNA clone H3024F06 3' read Polyadenylation signal
  BF450303 cDNA clone IMAGE:3674487 spontaneous tumor, metastatic to mammary. stem cell origin. 3' read Polyadenylation signal
  BI158056 cDNA clone IMAGE:5061305 tumor, gross tissue 5' read Polyadenylation signal

SENSE ESTs

  BI107798 cDNA clone IMAGE:5031460 tumor, gross tissue 5' read
  AW212227 cDNA clone IMAGE:2649211 tumor, gross tissue 3' read
  AA239881 cDNA clone IMAGE:692738 liver 5' read
  AI159323 cDNA clone IMAGE:1447878 mammary gland 5' read
  BG976450 cDNA clone IMAGE:4976608 infiltrating ductal carcinoma 5' read
  BI081401 cDNA clone IMAGE:5010788 tumor, biopsy sample 5' read
  BE448697 cDNA clone IMAGE:3331105 spleen 5' read
  BG923883 cDNA clone IMAGE:4953379 infiltrating ductal carcinoma 5' read
  AW211606 cDNA clone IMAGE:2649211 tumor, gross tissue 5' read
  BG918026 cDNA clone IMAGE:4948049 infiltrating ductal carcinoma 5' read
  BI108237 cDNA clone IMAGE:5031835 tumor, gross tissue 5' read