Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.196638
Description:Rab6, kinesin-like
Best-Of-UniGene (BOU) SequenceNM_009004
Genomic Coordiantes Displayed:Bases 4128638 to 4167365 of contig GA_x5J8B7W46SK
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.196638.jpeg
Best sense EST/protein match:BG245923 matched ref|NP_033030.1| (NM_009004) Rab6, kinesin-like; Rabkinesin-6 [Mus musculus] (E = e-114)
Best antisense EST/protein match:AA615957 matched pir||T51168 anaphase-promoting complex subunit 8 [imported] - human (E = e-123)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI987647 cDNA clone (no-name) neural retina
  BI986944 cDNA clone (no-name) neural retina
  BI989551 cDNA clone (no-name) neural retina
  AW762624 cDNA clone IMAGE:3155086 tumor, gross tissue 5' read Protein similarity
  BG808757 cDNA clone (no-name) neural retina
  BG801489 cDNA clone (no-name) neural retina
  AA770792 cDNA clone IMAGE:1162827 muscle 5' read Protein similarity
  BI106734 cDNA clone IMAGE:5036414 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI107589 cDNA clone IMAGE:5038904 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  AA615957 cDNA clone IMAGE:1066857 mammary gland 5' read Protein similarity
  BE373688 cDNA clone IMAGE:3584515 tumor, biopsy sample 5' read Protein similarity
  AA111385 cDNA clone IMAGE:555045 embryo 5' read Protein similarity
  AA162195 cDNA clone IMAGE:540804 embryo 5' read Protein similarity
  W64386 cDNA clone IMAGE:386408 embryo 5' read Protein similarity
  AA673921 cDNA clone IMAGE:1065684 muscle 5' read Protein similarity
  BI690087 cDNA clone IMAGE:5346615 infiltrating ductal carcinoma 5' read Protein similarity
  W78574 cDNA clone IMAGE:401997 embryo 5' read Protein similarity
  AA008492 cDNA clone IMAGE:439627 embryo 5' read Protein similarity
  W56906 cDNA clone IMAGE:368636 embryo 5' read Protein similarity
  BG086599 cDNA clone H3128C08 5' read Protein similarity
  BI693266 cDNA clone IMAGE:5371480 tumor, biopsy sample 5' read Protein similarity
  BG073882 cDNA clone H3128C08 3' read Protein similarityPolyadenylation signal
  AW551823 cDNA clone L0204C07 3' read Protein similarityPolyadenylation signal
  BG061556 cDNA clone L0907H12 5' read
  AL363702 cDNA clone R9306B26 embryo 5' read
  BG061499 cDNA clone L0907C09 5' read
  BG085455 cDNA clone H3113E01 5' read
  AA033473 cDNA clone IMAGE:466234 embryo 5' read
  W98411 cDNA clone IMAGE:424359 embryo 5' read
  AA657164 cDNA clone IMAGE:1121785 muscle 5' read
  BE533653 cDNA clone IMAGE:3598775 infiltrating ductal carcinoma 5' read
  BI736751 cDNA clone IMAGE:5367445 retina 5' read
  AW763707 cDNA clone IMAGE:3155086 tumor, gross tissue 3' read
  AI480782 cDNA clone IMAGE:821347 embryo 3' read
  AI552352 cDNA clone IMAGE:821347 embryo 5' read
  BI664719 cDNA clone IMAGE:5324017 infiltrating ductal carcinoma 5' read
  AA273704 cDNA clone IMAGE:764732 spleen 5' read
  BI651090 cDNA clone IMAGE:5337900 tumor, gross tissue 5' read Polyadenylation signal
  BG060796 cDNA clone L0907H12 3' read Polyadenylation signal
  BG060743 cDNA clone L0907C09 3' read Polyadenylation signal
  AW546409 cDNA clone L0007H12 3' read Polyadenylation signal
  BG072661 cDNA clone H3113E01 3' read Polyadenylation signal
  AA437844 cDNA clone IMAGE:821347 embryo 5' read
  AW541772 cDNA clone C0149D04 3' read Polyadenylation signal
  BG229934 cDNA clone IMAGE:4000621 spleen 3' read Polyadenylation signal
  AI154333 cDNA clone IMAGE:1447532 mammary gland 5' read Protein similarity
  BE532555 cDNA clone IMAGE:3597755 infiltrating ductal carcinoma 5' read Protein similarity

SENSE ESTs

  AL024348 cDNA clone 528-7I11 dissected endoderm 5' read Protein similarityPolyadenylation signal
  AI447737 cDNA clone IMAGE:599681 embryo 3' read Protein similarity
  AA007960 cDNA clone IMAGE:438008 embryo 5' read Protein similarity
  W80178 cDNA clone IMAGE:402871 embryo 5' read Protein similarity
  W59079 cDNA clone IMAGE:374094 embryo 5' read Protein similarity
  AA272723 cDNA clone IMAGE:734582 embryo 5' read Protein similarity
  AA389390 cDNA clone IMAGE:599681 embryo 5' read
  AA231338 cDNA clone IMAGE:672903 embryo 5' read Protein similarityPolyadenylation signal
  AI155804 cDNA clone IMAGE:1479613 uterus 5' read Protein similarity
  AI595871 cDNA clone IMAGE:1969463 embryo 3' read Protein similarityPolyadenylation signal
  AU022878 cDNA clone J0421H06 unfertilized egg 3' read Protein similarityPolyadenylation signal
  C76728 cDNA clone J0017H09 embryo 3' read Protein similarityPolyadenylation signal
  AW546437 cDNA clone L0008C07 3' read Protein similarityPolyadenylation signal
  C76591 cDNA clone J0014D08 embryo 3' read Protein similarityPolyadenylation signal
  AA771048 cDNA clone IMAGE:1163197 muscle 5' read Protein similarity
  AI048103 cDNA clone IMAGE:1022045 embryo 5' read Protein similarity
  AI595875 cDNA clone IMAGE:1969476 embryo 3' read Protein similarityPolyadenylation signal
  AW208960 cDNA clone IMAGE:2581916 infiltrating ductal carcinoma 3' read Protein similarityPolyadenylation signal
  AA048156 cDNA clone IMAGE:477072 embryo 5' read Protein similarity
  AA042071 cDNA clone IMAGE:474815 embryo 5' read Protein similarity
  C76864 cDNA clone J0021F01 embryo 3' read Protein similarityPolyadenylation signal
  BF226082 cDNA clone IMAGE:3672235 infiltrating ductal carcinoma 3' read Protein similarityPolyadenylation signal
  AA039103 cDNA clone IMAGE:474719 embryo 5' read Protein similarity
  BE570123 cDNA clone IMAGE:3709383 infiltrating ductal carcinoma 5' read Protein similarity
  AA003916 cDNA clone IMAGE:439238 embryo 5' read Protein similarity
  BE371199 cDNA clone IMAGE:3587764 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BG921160 cDNA clone IMAGE:4951598 infiltrating ductal carcinoma 5' read Protein similarity
  BG245923 cDNA clone IMAGE:4487192 tumor, biopsy sample 5' read Protein similarity
  AA589012 cDNA clone IMAGE:908432 embryo 5' read Protein similarity
  BI659797 cDNA clone IMAGE:5348856 tumor, gross tissue 5' read Protein similarity
  BI691503 cDNA clone IMAGE:5346903 infiltrating ductal carcinoma 5' read Protein similarity
  BI558150 cDNA clone IMAGE:5293144 tumor, gross tissue 5' read Protein similarity
  BI688099 cDNA clone IMAGE:5354417 infiltrating ductal carcinoma 5' read Protein similarity
  BI664991 cDNA clone IMAGE:5323549 infiltrating ductal carcinoma 5' read Protein similarity
  AA177197 cDNA clone IMAGE:633209 embryonal carcinoma 5' read Protein similarity
  BE569819 cDNA clone IMAGE:3709128 infiltrating ductal carcinoma 5' read Protein similarity
  BI659582 cDNA clone IMAGE:5348744 tumor, gross tissue 5' read Protein similarity
  BI653942 cDNA clone IMAGE:5324566 tumor, gross tissue 5' read Protein similarity
  BG974265 cDNA clone IMAGE:4979643 tumor, gross tissue 5' read Protein similarity
  BF731106 cDNA clone IMAGE:3976693 marginal zone b-cell tumor 5' read Protein similarity
  BE912171 cDNA clone IMAGE:3963804 tumor, biopsy sample 5' read Protein similarity
  BF167488 cDNA clone IMAGE:3995733 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BG919240 cDNA clone IMAGE:4946971 infiltrating ductal carcinoma 5' read Protein similarity
  AW212997 cDNA clone IMAGE:2581916 infiltrating ductal carcinoma 5' read Protein similarity
  BF226116 cDNA clone IMAGE:3672235 infiltrating ductal carcinoma 5' read Protein similarity
  AI574320 cDNA clone IMAGE:1969476 embryo 5' read Protein similarity
  AI574316 cDNA clone IMAGE:1969463 embryo 5' read Protein similarity