Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.20848
Description:regulatory factor X-associated ankyrin-containing protein
Best-Of-UniGene (BOU) SequenceNM_011266
Genomic Coordiantes Displayed:Bases 508000 to 522000 of contig GA_x5J8B7W3T6H
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.20848.jpeg
Best sense EST/protein match:BE633228 matched gb|AAH10971.1|AAH10971 (BC010971) Similar to regulatory factor X-associated (E = e-107)
Best antisense EST/protein match:BG914520 matched ref|XP_072027.1| (XM_072027) hypothetical protein XP_072027 [Homo sapiens] (E = 5e-56)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG071925 cDNA clone H3104G03 3' read Polyadenylation signal
  AU045417 cDNA clone J0934E01 3' read Polyadenylation signal
  BG074649 cDNA clone H3137F06 3' read
  AW553891 cDNA clone L0233B06 3' read
  AI875441 cDNA clone IMAGE:1972234 kidney 3' read
  BI649935 cDNA clone IMAGE:5337213 tumor, gross tissue 5' read Protein similarity
  BI788571 cDNA clone (no-name) pancreas 3' read Protein similarity
  BF785079 cDNA clone IMAGE:4236848 kidney 5' read
  AA222666 cDNA clone IMAGE:661119 embryo 5' read
  AI464430 cDNA clone IMAGE:661119 embryo 3' read
  BG077353 cDNA clone H3014E04 5' read
  BF179479 cDNA clone IMAGE:4038277 tumor, gross tissue 5' read
  BF164356 cDNA clone IMAGE:3992105 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI654442 cDNA clone IMAGE:5325363 tumor, gross tissue 5' read
  BG864055 cDNA clone IMAGE:4918808 tumor, gross tissue 5' read
  BG914520 cDNA clone IMAGE:4935545 tumor, gross tissue 5' read
  BG921200 cDNA clone IMAGE:4954275 infiltrating ductal carcinoma 5' read
  BI153621 cDNA clone IMAGE:5003230 tumor, biopsy sample 5' read
  AI785106 cDNA clone IMAGE:1888310 liver 5' read
  AA617461 cDNA clone IMAGE:934932 embryo 5' read
  BE336172 cDNA clone IMAGE:3325244 spleen 5' read
  BG915413 cDNA clone IMAGE:4945246 tumor, gross tissue 5' read
  AA175333 cDNA clone IMAGE:617909 spleen 5' read
  AA111465 cDNA clone IMAGE:557361 embryo 5' read Polyadenylation signal
  AI255978 cDNA clone IMAGE:1888310 liver 3' read Polyadenylation signal
  BG807481 cDNA clone (no-name) neural retina
  AW319126 cDNA clone IMAGE:2372922 kidney 3' read
  AI467574 cDNA clone IMAGE:820446 mammary gland 3' read
  AI608427 cDNA clone IMAGE:946410 embryo 3' read
  BB661757 cDNA clone D730026H12 mammary gland
  AA528957 cDNA clone IMAGE:905261 colon 5' read

SENSE ESTs

  AI462805 cDNA clone IMAGE:762624 embryo 3' read Polyadenylation signal
  BI714182 cDNA clone (no-name) pancreas 3' read Protein similarityPolyadenylation signal
  BB622691 cDNA clone 6430524H01 olfactory brain 5' read
  AI504262 cDNA clone IMAGE:962866 embryo 3' read
  AW212534 cDNA clone IMAGE:2598919 infiltrating ductal carcinoma 3' read
  AI553301 cDNA clone IMAGE:905261 colon 5' read
  AI595048 cDNA clone IMAGE:762624 embryo 5' read
  AA271206 cDNA clone IMAGE:762624 embryo 5' read
  W54073 cDNA clone IMAGE:368138 embryo 5' read
  BG803271 cDNA clone (no-name) neural retina
  BE686312 cDNA clone IMAGE:3412913 spleen 3' read Protein similarityPolyadenylation signal
  BI730859 cDNA clone IMAGE:5359314 retina 5' read Protein similarity
  BE633228 cDNA clone IMAGE:3412913 spleen 5' read Protein similarity
  BG963031 cDNA clone IMAGE:4982675 colon 5' read Protein similarity
  BI686210 cDNA clone IMAGE:5352674 infiltrating ductal carcinoma 5' read Protein similarity
  BF539058 cDNA clone IMAGE:4190691 salivary gland 5' read Protein similarity
  BI714453 cDNA clone (no-name) pancreas 5' read Protein similarity
  AA616119 cDNA clone IMAGE:1066950 mammary gland 5' read Protein similarity
  AA259432 cDNA clone IMAGE:733633 embryo 5' read Protein similarity
  BG087273 cDNA clone H3137F06 5' read