Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.24044
Description:beta-site APP cleaving enzyme
Best-Of-UniGene (BOU) SequenceNM_011792
Genomic Coordiantes Displayed:Bases 1427800 to 1436200 of contig GA_x5J8B7W3663
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.24044.jpeg
Best sense EST/protein match:BI157614 matched ref|NP_035922.2| (NM_011792) beta-site APP cleaving enzyme; APP beta-secretase [Mus (E = e-107)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF452822 cDNA clone IMAGE:3821447 spleen 5' read Protein similarity
  BI076445 cDNA clone J1015E10 3' read
  BG072311 cDNA clone H3109B04 3' read
  BG085145 cDNA clone H3109B04 5' read
  BE382037 cDNA clone IMAGE:3601247 tumor, biopsy sample 5' read
  AA980643 cDNA clone IMAGE:1349538 mammary gland 5' read
  AA239258 cDNA clone IMAGE:698147 pool 5' read
  AI466745 cDNA clone IMAGE:698147 pool 5' read Protein similarity
  BG804641 cDNA clone (no-name) neural retina
  BG872141 cDNA clone IMAGE:4924243 salivary gland 5' read Protein similarity
  AW551394 cDNA clone L0078B08 3' read Protein similarity

SENSE ESTs

  BI499122 cDNA clone (no-name) pancreas 3' read
  BI738422 cDNA clone IMAGE:5368270 retina 5' read
  BE532500 cDNA clone IMAGE:3597738 infiltrating ductal carcinoma 5' read
  BI328761 cDNA clone IMAGE:5137666 liver 5' read
  AI892003 cDNA clone IMAGE:2123230 kidney 5' read
  BG972294 cDNA clone IMAGE:4975446 kidney 5' read
  BF322492 cDNA clone IMAGE:3812294 liver 3' read
  AU014775 cDNA clone J0702E11 embryo 3' read
  BF449917 cDNA clone IMAGE:3813966 brain 3' read
  BF135513 cDNA clone IMAGE:4012852 tumor, metastatic to mammary 5' read
  BM052274 cDNA clone (no-name) 3' read Polyadenylation signal
  BM052237 cDNA clone (no-name) 3' read Polyadenylation signal
  BG069998 cDNA clone H3083E02 3' read
  BG083012 cDNA clone H3083E02 5' read
  AA796738 cDNA clone IMAGE:1069079 mammary gland 5' read
  BI499374 cDNA clone (no-name) pancreas 5' read
  BM052494 cDNA clone (no-name) 5' read
  BM052555 cDNA clone (no-name) 5' read
  AW240686 cDNA clone IMAGE:2811051 tumor, gross tissue 3' read Polyadenylation signal
  BF660066 cDNA clone IMAGE:3812294 liver 5' read
  BE374585 cDNA clone IMAGE:3583744 tumor, biopsy sample 5' read
  BF449077 cDNA clone IMAGE:3813966 brain 5' read
  BF144169 cDNA clone IMAGE:4016632 tumor, metastatic to mammary 5' read Protein similarity
  BF120956 cDNA clone IMAGE:3987065 tumor, gross tissue 5' read
  BB609625 cDNA clone 1110027D02 5' read
  BE572081 cDNA clone IMAGE:3711490 infiltrating ductal carcinoma 5' read Protein similarity
  AW258234 cDNA clone IMAGE:2811051 tumor, gross tissue 5' read Protein similarity
  BI157614 cDNA clone IMAGE:5062869 tumor, gross tissue 5' read Protein similarity