Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.31239
Description:expressed sequence AW107933
Best-Of-UniGene (BOU) SequenceAV055905
Genomic Coordiantes Displayed:Bases 13119891 to 13160568 of contig GA_x5J8B7W4QPD
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.31239.jpeg
Best sense EST/protein match:BI734057 matched gb|AAF08683.1|AF107723_1 (AF107723) activator of G-protein signaling 3 [Rattus norvegicus] (E = 6e-79)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  W88133 cDNA clone IMAGE:407558 embryo 5' read
  BG962193 cDNA clone IMAGE:4981695 colon 5' read
  AI596768 cDNA clone IMAGE:949330 mammary gland 5' read
  BF607732 cDNA clone ICRFp522I2458 whole embryo 5' read
  AA261754 cDNA clone IMAGE:734752 embryo 5' read
  AA542018 cDNA clone IMAGE:949330 mammary gland 5' read
  AA444701 cDNA clone IMAGE:832009 mammary gland 5' read
  AA733368 cDNA clone IMAGE:1176857 colon 5' read
  AI875001 cDNA clone IMAGE:2099599 kidney 3' read Polyadenylation signal
  BE303494 cDNA clone IMAGE:3500442 infiltrating ductal carcinoma 5' read Polyadenylation signal
  AI503643 cDNA clone IMAGE:949321 mammary gland 3' read Polyadenylation signal
  BE532076 cDNA clone IMAGE:3599493 infiltrating ductal carcinoma 5' read
  BI966382 cDNA clone (no-name) 3' read Polyadenylation signal
  BM022112 cDNA clone (no-name) 3' read Polyadenylation signal
  AI626988 cDNA clone IMAGE:1152968 muscle 3' read

SENSE ESTs

  BI733501 cDNA clone IMAGE:5359839 retina 5' read
  BE226010 cDNA clone (no-name) pancreatic bud 3' read Polyadenylation signal
  BI730021 cDNA clone IMAGE:5357119 retina 5' read Polyadenylation signal
  BF450049 cDNA clone IMAGE:3813803 brain 3' read Polyadenylation signal
  BI440048 cDNA clone (no-name) 3' read Polyadenylation signal
  AA271904 cDNA clone IMAGE:736843 embryo 5' read Protein similarity
  AW107933 cDNA clone IMAGE:2225516 embryo 3' read Polyadenylation signal
  W44240 cDNA clone IMAGE:353882 embryo 5' read
  AA050515 cDNA clone IMAGE:476885 embryo 5' read
  AA036169 cDNA clone IMAGE:468857 embryo 5' read
  BG806078 cDNA clone (no-name) neural retina
  BF578637 cDNA clone IMAGE:4207406 colon 5' read
  BF302294 cDNA clone IMAGE:4166296 salivary gland 5' read Protein similarity
  BF449651 cDNA clone IMAGE:3813803 brain 5' read Protein similarity
  BG695688 cDNA clone IMAGE:4318189 pituitary gland 5' read Protein similarity
  BI440474 cDNA clone (no-name) 5' read Protein similarity
  BB610371 cDNA clone 1810037C22 pancreas 5' read Protein similarity
  BE533188 cDNA clone IMAGE:3599422 infiltrating ductal carcinoma 5' read Protein similarity
  BG261774 cDNA clone IMAGE:4480816 retina 5' read Protein similarity
  BG976952 cDNA clone IMAGE:4976495 infiltrating ductal carcinoma 5' read Protein similarity
  BB578095 cDNA clone 6430400L12 olfactory brain 5' read
  BG062466 cDNA clone L0953A10 5' read Protein similarity
  BI734057 cDNA clone IMAGE:5363182 retina 5' read Protein similarity
  BG297299 cDNA clone IMAGE:4506917 retina 5' read Protein similarity
  BI735318 cDNA clone IMAGE:5364215 retina 5' read Protein similarity
  BI736135 cDNA clone IMAGE:5366591 retina 5' read Protein similarity
  BG294972 cDNA clone IMAGE:4504055 retina 5' read Protein similarity
  AW318405 cDNA clone IMAGE:2352594 embryo 5' read Protein similarity