Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.32800
Description:RIKEN cDNA 4932411A20 gene
Best-Of-UniGene (BOU) SequenceAK019855
Genomic Coordiantes Displayed:Bases 10965800 to 11058200 of contig GA_x5J8B7W7SQR
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.32800.jpeg
Best sense EST/protein match:AK019855 matched dbj|BAB71035.1| (AK055884) unnamed protein product [Homo sapiens] (E = e-110)
Best antisense EST/protein match:BG920894 matched dbj|BAB71617.1| (AK057940) unnamed protein product [Homo sapiens] (E = 2e-38)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG806153 cDNA clone (no-name) neural retina
  BE532537 cDNA clone IMAGE:3597749 infiltrating ductal carcinoma 5' read
  BI685995 cDNA clone IMAGE:5344396 infiltrating ductal carcinoma 5' read Protein similarity
  BE303861 cDNA clone IMAGE:3499552 infiltrating ductal carcinoma 5' read
  AA120427 cDNA clone IMAGE:537403 embryo 5' read
  BG920894 cDNA clone IMAGE:4953838 infiltrating ductal carcinoma 5' read

SENSE ESTs

  AW105790 cDNA clone IMAGE:2236294 kidney 3' read Polyadenylation signal
  AV286711 cDNA clone 5031426I22 ovary and uterus
  AW558425 cDNA clone L0296C02 3' read Polyadenylation signal
  BG076238 cDNA clone H3157C07 3' read Polyadenylation signal
  W90842 cDNA clone IMAGE:420456 embryo 5' read
  AA050035 cDNA clone IMAGE:478509 embryo 5' read
  BG088763 cDNA clone H3157C07 5' read
  BE570611 cDNA clone IMAGE:3707691 infiltrating ductal carcinoma 5' read
  BF451655 cDNA clone IMAGE:3675110 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF160110 cDNA clone IMAGE:3983955 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BE572773 cDNA clone IMAGE:3602111 infiltrating ductal carcinoma 5' read
  AW106724 cDNA clone IMAGE:2236294 kidney 5' read Protein similarity