Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.35867
Description:cyclin E2
Best-Of-UniGene (BOU) SequenceAK010929
Genomic Coordiantes Displayed:Bases 1649359 to 1698111 of contig GA_x5J8B7W3DV4
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.35867.jpeg
Best sense EST/protein match:AK010929 matched ref|NP_033960.1| (NM_009830) cyclin E 2 [Mus musculus] (E = 0.0)
Best antisense EST/protein match:BI558991 matched ref|XP_005194.5| (XM_005194) hypothetical protein FLJ20530 [Homo sapiens] (E = e-102)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA644799 cDNA clone IMAGE:1152794 muscle 5' read
  BI558991 cDNA clone IMAGE:5294389 tumor, gross tissue 5' read
  BF167436 cDNA clone IMAGE:3995433 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AW536492 cDNA clone G0105B05 3' read Protein similarityPolyadenylation signal
  BF227541 cDNA clone IMAGE:3670572 tumor, gross tissue 3' read Protein similarityPolyadenylation signal
  BG244804 cDNA clone IMAGE:4484886 tumor, biopsy sample 5' read
  BG244717 cDNA clone IMAGE:4484933 tumor, biopsy sample 5' read Protein similarityPolyadenylation signal
  BG965661 cDNA clone IMAGE:4985089 colon 5' read
  AU024668 cDNA clone J0458B03 unfertilized egg 3' read
  AA980243 cDNA clone IMAGE:1348403 mammary gland 5' read Protein similarity
  BG965660 cDNA clone IMAGE:4985041 colon 5' read Protein similarityPolyadenylation signal
  AA274196 cDNA clone IMAGE:764789 spleen 5' read
  BF140740 cDNA clone IMAGE:4014815 tumor, metastatic to mammary 5' read Protein similarity
  BF101939 cDNA clone IMAGE:3980569 tumor, biopsy sample 5' read
  AI626975 cDNA clone IMAGE:1152794 muscle 3' read Protein similarityPolyadenylation signal
  BB506509 cDNA clone D730008I04 mammary gland
  BB509926 cDNA clone D730026O07 mammary gland Protein similarity
  BB512560 cDNA clone D730040J05 mammary gland
  BB505580 cDNA clone D730003K12 mammary gland
  BB512412 cDNA clone D730039N17 mammary gland

SENSE ESTs

  AW544792 cDNA clone C0186A01 3' read
  BE374053 cDNA clone IMAGE:3586151 tumor, biopsy sample 5' read
  BI696437 cDNA clone IMAGE:5373281 tumor, biopsy sample 5' read
  BG918215 cDNA clone IMAGE:4948864 infiltrating ductal carcinoma 5' read Protein similarity
  AW321767 cDNA clone IMAGE:2631788 infiltrating ductal carcinoma 5' read Protein similarity
  BE368876 cDNA clone IMAGE:3590427 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI687952 cDNA clone IMAGE:5355771 infiltrating ductal carcinoma 5' read Protein similarity
  BE304094 cDNA clone IMAGE:3499789 infiltrating ductal carcinoma 5' read Protein similarity
  AW258269 cDNA clone IMAGE:2811097 tumor, gross tissue 5' read Protein similarity