Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.4358
Description:mitogen activated protein kinase kinase kinase 12
Best-Of-UniGene (BOU) SequenceNM_009582
Genomic Coordiantes Displayed:Bases 11820397 to 11836058 of contig GA_x5J8B7W76D0
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.4358.jpeg
Best sense EST/protein match:AW258670 matched ref|NP_033608.1| (NM_009582) zipper (leucine) protein kinase [Mus musculus] (E = e-101)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA870820 cDNA clone IMAGE:1095417 5' read
  AA473712 cDNA clone IMAGE:866157 embryo 5' read
  AA212840 cDNA clone IMAGE:677201 liver 5' read
  AA546328 cDNA clone IMAGE:945650 embryo 5' read
  AA000178 cDNA clone IMAGE:425631 embryo 5' read
  BI251411 cDNA clone IMAGE:5150264 tumor, gross tissue 5' read
  W91034 cDNA clone IMAGE:421154 embryo 5' read
  BE287853 cDNA clone IMAGE:3497296 tumor, gross tissue 5' read
  BF152095 cDNA clone IMAGE:3670064 tumor, gross tissue 5' read
  AA008028 cDNA clone IMAGE:438406 embryo 5' read
  AV285900 cDNA clone 5031415E16 ovary and uterus
  BF319905 cDNA clone IMAGE:3665728 tumor, gross tissue 3' read
  W54492 cDNA clone IMAGE:367895 embryo 5' read
  BF227461 cDNA clone IMAGE:3670064 tumor, gross tissue 3' read
  AW556977 cDNA clone L0275G08 3' read
  BG075727 cDNA clone H3150H08 3' read
  BF149845 cDNA clone IMAGE:3665728 tumor, gross tissue 5' read
  BI697694 cDNA clone IMAGE:5376475 tumor, biopsy sample 5' read
  BG083632 cDNA clone H3090D01 5' read Protein similarity

SENSE ESTs

  BG807373 cDNA clone (no-name) neural retina
  AU016865 cDNA clone J0731A03 embryo 3' read Protein similarity
  BG070715 cDNA clone H3090D01 3' read Protein similarity
  BI248600 cDNA clone IMAGE:5148680 tumor, gross tissue 5' read Protein similarity
  BI689652 cDNA clone IMAGE:5356163 infiltrating ductal carcinoma 5' read Protein similarity
  W97442 cDNA clone IMAGE:422079 embryo 5' read Protein similarity
  AU066756 cDNA clone MNCb-2527 brain 5' read Protein similarity
  AW258670 cDNA clone IMAGE:2811677 tumor, gross tissue 5' read Protein similarity
  BI156670 cDNA clone IMAGE:5061643 tumor, gross tissue 5' read Protein similarity
  BI739827 cDNA clone IMAGE:5369193 retina 5' read Protein similarity
  BI687731 cDNA clone IMAGE:5355872 infiltrating ductal carcinoma 5' read