Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.4390
Description:numb gene homolog (Drosophila)
Best-Of-UniGene (BOU) SequenceAK004553
Genomic Coordiantes Displayed:Bases 2562858 to 2741418 of contig GA_x5J8B7W42B8
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.4390.jpeg
Best sense EST/protein match:AK004553 matched dbj|BAB23367.1| (AK004553) data source:MGD, source key:MGI:107423, evidence:ISS~numb (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI157675 cDNA clone IMAGE:5060799 tumor, gross tissue 5' read
  BI154930 cDNA clone IMAGE:5032567 tumor, gross tissue 5' read Protein similarity
  BF140840 cDNA clone IMAGE:4014193 tumor, metastatic to mammary 5' read
  BI648512 cDNA clone IMAGE:5317942 tumor, gross tissue 5' read Polyadenylation signal
  BI158663 cDNA clone IMAGE:5061993 tumor, gross tissue 5' read Polyadenylation signal
  BI649584 cDNA clone IMAGE:5319804 tumor, gross tissue 5' read
  BF134902 cDNA clone IMAGE:4007929 tumor, metastatic to mammary 5' read
  BI651912 cDNA clone IMAGE:5339147 tumor, gross tissue 5' read
  BI652882 cDNA clone IMAGE:5340652 tumor, gross tissue 5' read Protein similarity
  BI651723 cDNA clone IMAGE:5339257 tumor, gross tissue 5' read
  AA959389 cDNA clone IMAGE:1347084 mammary gland 5' read

SENSE ESTs

  BF608526 cDNA clone ICRFp522G0275 whole embryo 5' read
  BG962964 cDNA clone IMAGE:4982964 colon 5' read Protein similarity
  BF164693 cDNA clone IMAGE:3991630 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI219648 cDNA clone IMAGE:5098978 liver 5' read Protein similarity
  BF785940 cDNA clone IMAGE:4240578 kidney 5' read Protein similarity
  BG803374 cDNA clone (no-name) neural retina
  BI148299 cDNA clone IMAGE:5055434 liver 5' read
  AW215292 cDNA clone IMAGE:2651299 tumor, metastatic to mammary 5' read Protein similarity
  BF179738 cDNA clone IMAGE:4037217 tumor, gross tissue 5' read Protein similarity
  BG804472 cDNA clone (no-name) neural retina
  AA544911 cDNA clone IMAGE:949254 mammary gland 5' read Protein similarity
  BI695715 cDNA clone IMAGE:5372610 tumor, biopsy sample 5' read Protein similarity
  W36025 cDNA clone IMAGE:353083 embryo 5' read
  BG921782 cDNA clone IMAGE:4951760 infiltrating ductal carcinoma 5' read
  AA967084 cDNA clone IMAGE:1349165 mammary gland 5' read
  AA035815 cDNA clone IMAGE:466066 embryo 5' read
  BI737432 cDNA clone IMAGE:5365155 retina 5' read
  AA261253 cDNA clone IMAGE:747864 liver 5' read
  AA290535 cDNA clone IMAGE:749091 spleen 5' read
  AI155458 cDNA clone IMAGE:1477726 uterus 5' read
  AW552020 cDNA clone L0207B06 3' read Polyadenylation signal
  AW544603 cDNA clone C0183F10 3' read Polyadenylation signal
  AW544595 cDNA clone C0183F02 3' read Polyadenylation signal
  BF016688 cDNA clone IMAGE:3661458 tumor, metastatic to mammary 5' read
  BE686646 cDNA clone IMAGE:3415584 spleen 3' read
  AW552031 cDNA clone L0207C12 3' read Polyadenylation signal
  AA606617 cDNA clone IMAGE:1005484 embryo 5' read
  AA466882 cDNA clone IMAGE:834682 embryo 5' read