Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.45165
Description:RIKEN cDNA 2310040B03 gene
Best-Of-UniGene (BOU) SequenceAK009713
Genomic Coordiantes Displayed:Bases 1928238 to 1931568 of contig GA_x5J8B7W3S0S
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.45165.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:BI991111 matched gb|AAH20110.1|AAH20110 (BC020110) Unknown (protein for MGC:28368) [Mus musculus] (E = 4e-47)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF582733 cDNA clone IMAGE:4208268 colon 5' read
  BI991111 cDNA clone (no-name) neural retina
  BF540443 cDNA clone IMAGE:4191866 salivary gland 5' read
  BF659540 cDNA clone IMAGE:3812811 liver 5' read

SENSE ESTs

  BB563246 cDNA clone 1100001M23 5' read
  BI250884 cDNA clone IMAGE:5148988 tumor, gross tissue 5' read
  BE633114 cDNA clone IMAGE:3412787 spleen 5' read
  AI510194 cDNA clone IMAGE:474711 embryo 5' read
  AA039005 cDNA clone IMAGE:474711 embryo 5' read