Information Item | Value |
---|---|
Dataset Name | Mye_gal: MED2 mutant galactose array |
Dataset Number | 27 |
Short Description | MED2 deletion strain effect when grown on galactose |
Source URL | http://cmgm.stanford.edu/pbrown/med2/ |
Reference | Lawrence C. Myers, Claes M. Gustafsson, Kathleen C. Hayashibara, Patrick O. Brown, and Roger D. Kornberg. Proc. Natl. Acad. Sci, USA. Vol. 96, pp 67-72 (1999). |
Strains | Wild type strain MG106 is Red, med2 deletion strain MG107 is green |
Conditions | galactose, companion data set with heat shock loaded as separate dataset |
Date Added to ExpressDB | May 19 1999 12:00:45:546PM |
Number of Measures on ExpressDB | 8 (here to download dataset and view measure details) |
Long Description | Medline abstract in progress: Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation in vivo were closely coincident with those in vitro. A whole genome expression profile of a Deltamed2 strain showed diminished transcription of a subset of inducible genes but only minor effects on 'basal' transcription. These findings make an important connection between transcriptional activation in vitro and in vivo, and identify Mediator as a 'global' transcriptional coactivator. |
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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University