George M. Church Laboratory
mRNA Abundance Home Page
Welcome to the new and (as usual) evolving Church Lab home page for whole genome mRNA abundance studies.
The purpose of this page is to share the mRNA abundance data that our lab has generated, as well as software tools for data analysis.
At present, this site contains only data and tools developed in the context of a recent article:
Finding DNA Regulatory Motifs within Unaligned Non-Coding Sequences Clustered by Whole-Genome mRNA Quantitation, Roth, F.R., Hughes, J. D., Estep, P. E. & G.M. Church, Nature Biotechnology 1998
This article describes a method for discovery of DNA motifs which regulate gene transcription, using mRNA abundance data and a computer. This approach does not require prior information about transcription factors or their binding sites.
Briefly the approach is:
The data from these four experiments can be found here.
Protocols used in these experiments can be found here.
AlignACE software, which Aligns Nucleic Acid Conserved Elements, as well as other software for analysis of mRNA data, can be found here
Please direct any questions or comments to Jason Hughes or Fritz Roth.
This page was last modified 3 Oct 98 by FR.