Global RNA half-life analysis in Escherichia coli reveals positional patterns of transcript degradation

Douglas W. Selinger*†, Rini Mukherjee Saxena*‡, Kevin J. Cheung†, George M. Church†, and Carsten Rosenow§‡

*These authors contributed equally to this work
§To whom correspondence should be addressed
†Harvard Medical School, Department of Genetics, Boston, MA 02115
‡Affymetrix Inc., Santa Clara, CA 95051

Affymetrix E. coli Microarray
Global RNA expression before rifampicin addition

(click here for higher resolution image - 387K )

Processed Data

-> 2,679 mRNA half-lives (non-linear least squares curvefitting), tab-delimited data , info
-> mRNA half-lives (raw average differences, HL calculation by 2-fold method), tab-delimited (Table 1, Figure 4)
-> Average operon half-lives , includes 5'-3' positional patterns, tab-delimited (Figure 1, Table 2)
-> Operon degradation pattern clustering (Figure 2)
        Treeview  formats: cdt file , gtr file
        Viewing formats: pdf file , ps file
        Data: Tab-delimited ranks
-> High-resolution operon degradation file , tab-delimited (Figure 3)
-> High-resolution transcript degradation (probe by probe, window size = 5 probes, tab-delimited)
    - watson strand (20 MB)
    - crick strand (17 MB)

Raw Data
-> .CEL files

-> GAPS©
-> Cluster/TreeView