(Protein) Purification Methods

• Charge: ion-exchange chromatography, isoelectric focusing

• Size: dialysis, gel-filtration chromatography,

gel-electrophoresis, sedimentation velocity

• Solubility: salting out

• Hydrophobicity: Reverse phase chromatography

• Specific binding: affinity chromatography

• Complexes: Immune precipitation (± crosslinking)

• Density: sedimentation equilibrium

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