Copy Number Control Models
Replication of ColE1 & R1 Plasmids
Determine the factors that govern the plasmid copy number
- cellular growth rate
- precise nature is not completely clear
- One way to address this question is via the use of a kinetic analysis of the replication process, and relate copy number to overall cellular growth.
Why? the copy number can be an important determinant of cloned protein production in recombinant microorganisms