ColE1 CNC
Mechanism:
- RNA II ? 555-bp RNA pre-primer
- transcribed upstream of OriC.
- RNase H ? cleaves the transcript near OriC
- no Rnase H ? RNA II transcription proceeds past OriC ? no replication
- The cleaved RNAII forms a primer for DNA replication
- RNA I can bind to RNA II and prevents RNase from cleaving RNA II
- Rom protein increases RNA I – RNA II interaction
- Saturated – increasing Rom protein does not decrease copy number