| Information Item | Value |
|---|---|
| Dataset Name | Spe_summary: Spellman-processed summary of cell cycle series ratios |
| Dataset Number | 19 |
| Short Description | data from approximately 60 timepoints, with series following alpha factor arrest, elutriation, and arrest of cdc15 temperature sensitive mutant |
| Source URL | http://cellcycle-www.stanford.edu/ |
| Reference | Spellman, Paul T. et al, Mol.Biol.Cell 9:3273 (1998) |
| Strains | DBY7286, DBY8724, W303a, #31, #245, and cdc15-2 |
| Conditions | Alpha-factor G1 release series, series following elutriation of small G1 cells, temp sensitive cdc15 mutant series following late M release, Cln3 and Clb2 induction experiments |
| Date Added to ExpressDB | Feb 23 1999 3:55:45:306PM |
| Number of Measures on ExpressDB | 77 (here to download dataset and view measure details) |
| Long Description | This is the master dataset combining all the ratios from the individual microarray datasets, which are not being loaded initially. It also includes data from Cho, Campbell, et. al., "A Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle", Molecular Cell (2), pp.65-73 (1998) Abstract from article: We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: alpha factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle-regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu/ |
Please contact Wayne Rindone for more information, or with any questions, comments, or concerns.
Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University