Expression Data Set 19 details

Information Item Value
Dataset Name Spe_summary: Spellman-processed summary of cell cycle series ratios
Dataset Number 19
Short Description data from approximately 60 timepoints, with series
following alpha factor arrest, elutriation, and arrest of cdc15 temperature
sensitive mutant
Source URL
Reference Spellman, Paul T. et al, Mol.Biol.Cell 9:3273 (1998)
Strains DBY7286, DBY8724, W303a, #31, #245, and cdc15-2
Conditions Alpha-factor G1 release series, series following elutriation
of small G1 cells, temp sensitive cdc15 mutant series following late M
release, Cln3 and Clb2 induction experiments
Date Added to ExpressDB Feb 23 1999 3:55:45:306PM
Number of Measures on ExpressDB 77 (here to download dataset and view measure details)
Long Description This is the master dataset combining all the ratios
from the individual microarray datasets, which are not being loaded
initially. It also includes data from Cho, Campbell, et. al., "A
Genome-Wide Transcriptional Analysis of the Mitotic Cell Cycle",
Molecular Cell (2), pp.65-73 (1998)

Abstract from article:

We sought to create a comprehensive catalog of yeast genes whose
transcript levels vary periodically within the cell cycle. To this
end, we used DNA microarrays and samples from yeast cultures
synchronized by three independent methods: alpha factor arrest,
elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using
periodicity and correlation algorithms, we identified 800 genes that
meet an objective minimum criterion for cell cycle regulation. In
separate experiments, designed to examine the effects of inducing
either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that
the mRNA levels of more than half of these 800 genes respond to one or
both of these cyclins. Furthermore, we analyzed our set of cell
cycle-regulated genes for known and new promoter elements and show
that several known elements (or variations thereof) contain
information predictive of cell cycle regulation. A full description
and complete data sets are available at

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Copyright (c) 2006 by Wayne Rindone and the President and Fellows of Harvard University