Machine Intelligence from Cortical Networks (MICrONS)
Intelligence Advanced Research Projects Activity (IARPA) Brain Research through Advancing Innovative Neurotechnologies. (BRAIN) BackgroundThe science behind Obama's BRAIN project. (BrainFacts, 15Apr-2013 | Jean-François Gariépy)
Wyss Institute Will Lead IARPA-Funded Brain Mapping Consortium (Wyss, 26-Jan-2016 |Benjamin Boettner, Seth Kroll)
Project Aims to Reverse-engineer Brain Algorithms, Make Computers Learn Like Humans (Scientific Computing, 4-Feb-2016 | Byron Spice)
The U.S. Government Launches a $100-Million "Apollo Project of the Brain" (Scientific American, 8-Mar-2016 | Jordana Cepelewicz) Grant Proposal
Tasks 2 & 3 PDF Harvard, Wyss, CSHL, MIT.
Task 1. CMU.
Related Projects:
Full Rosetta brains in situ
A. Activity (MICrONS = Ca imaging) (Alternative=Tickertape, see figure to right)
B. Behavior (MICrONS & Alt = traditional video)
C. Connectome (MICrONS & Alt = BOINC via Cas9-barcode)
D. Developmental Lineage (via Cas9-barcode)
E. Expression (RNA & Protein via FISSEQ) Building brain components, circuits and organoids.
Busskamp V, Lewis NE, Guye P, Ng AHM, Shipman S, Byrne SS, Sanjana NE, Li Y, Weiss R, Church GM (2014)
Rapid neurogenesis through transcriptional activation in human stem cells. Molecular Systems Biology MSB 10:760:1-21 Update 2025:
E11.bio (2023-present) FRO for fluorescent whole brain connectome
Connectomes.net (2023-present) Connectome applied to neurological disorders.
Eon.systems (2024-present) Connectomes for digital human intelligence.
Wang Y, Liu X, Zeng Y, Saka SK, Xie W, Goldaracena I, Kohman RE, Yin P, Church GM (2024) Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions. Nucleic Acids Res.
Goodwin DR, Vaughan A, Leible D, Alon S, Henry GL, Cheng A, Chen X, Zhang R, Xue AG, Wassie AT, Sinha A, Bando Y, Kajita A, Marblestone AH, Zador AM, Boyden ES, Church GM, Kohman RE (2022) Expansion Sequencing of RNA Barcoded Neurons in the Mammalian Brain: Progress and Implications for Molecularly Annotated Connectomics. Biorxiv.
Bhattarai-Kline S, Lear SK, Fishman CB, Lopez SC, Lockshin E, Schubert MG, Nivala J, Church G, Shipman SL (2022) Recording gene expression order in DNA by CRISPR addition of retron barcodes. Nature.
Kohman RE, Church GM (2020) Fluorescent in situ sequencing of DNA barcoded antibodies. Biorxiv.
Chen X, Sun YC, Church GM, Lee JH, Zador AM (2017) Efficient in situ barcode sequencing using padlock-probe-based BaristaSeq. Nucleic Acids Res. doi: 10.1093/nar/gkx1206.
