Stationary Phase of HPLC
The degree of interaction with samples determines the migration speed.
- Liquid-Solid: polarity.
- Liquid-Liquid: polarity.
- Size-Exclusion: porous beads.
- Normal Phase: hydrophilicity and lipophilicity.
- Reverse Phase: hydrophilicity and lipophilicity.
- Ion Exchange.
- Affinity: specific affinity.