RP-LC calculated observed

Empirical linear regression varies with type of

LC-material

a-NH3+? C18

no yes no

W 10.1 9.3 9.8

F 8.8 5.5 8.8

L 7.5 4.6 9.5

I 5.8 3.0 8.4

M 4.8 3.0 2.6

Y 4.5 3.1 6.1

V 3.5 1.3 4.9

C 3.4 2.9 0.5

P 2.7 0.7 2.8

E 0.3 0.5 0.8

A 0.2 0.1 1.7

D 0.0 0.6 1.1

G 0.0 0.0 0.4

T -0.1 1.0 1.8

S -0.8 -0.1 0.3

Q -0.9 0.0 -0.7

N -3.0 -2.1 0.0

R -3.1 -2.1 2.4

H -3.3 -1.5 0.6

K -3.5 -1.6 0.0

Sereda, T. et al. “Effect of the a-amino group on peptide retention behaviour in reversed-phase chromatography.

Wilce, et al. “High-performance liquid chromatography of amino acids, peptides and proteins.” Journal of Chromatography, 632 (1993) 11-18.

(The calculated curve is displaced upward for clarity)

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