Experimental Protocols (go back)

Polony Protocols

    Polony PCR Amplification

    Hybridization / Single Base Extension

    FISSEQ

    Bind Silane Treatment of Slides

    SYBR Green Staining

Buffer Recipes

    Wash 1E (aka WB2300 or Wash 1)

    Wash LS

    1x Klenow Extension Buffer

    6x SSPE w. Triton X-100

    1x Bleaching Buffer

    1x Alkylation Buffer

    1x Quenching Buffer

    TGT Solution

Stock Solutions [recipes can be found in the Maniatis manuals]

    2M KCl

    500 mM EDTA (pH 8.0)

    1M MgCl

    1M Tris-Acid

    1M Tris-Base

    20x SSC

    20x SSPE