DOE BioEnergy Technology Center at Harvard

1986 Abstract: Genomic Sequence Comparisons
The project objective is to implement new ideas for rapid, precise, and interpretable DNA sequencing. The approach consists of (1) multiplex sequencing, (2) subtractive sequencing, (3) comparisons between species, and (4) automation of filter hybridization, sequence data reading, and clone walking. Results include: (1) a full test of multiplexing, demonstrating nine reprobings without signal losses and sequencing 80 random oligonucleotides similar to those of the subtractive approach; and (2) a preliminary digital analysis of several sequence films. Expected results include nearly complete sequences for two bacterial genomes (10 Mbp total), estimates of the background levels of cross hybridizations in the subtractive and walking strategies, and a determination of the parameters that can minimize such effects.

1989 Abstract: Genomic Sequence Comparisons

1992 Abstract: Genomic Sequence Comparisons

1996 Proposal: Genomic Sequence Comparisons

1999 Proposal: New Technologies for Genome Sequencing and Annotation

May 2002 GTL Proposal: Microbial Ecology, Proteogenomics & Computational Optima
Introduction
Goal 1: Protein complexes & Mass Spectrometry
Goal 2: Regulatory Networks and RNA quantitation.
Goal 3: Microbial Communities, Tn tags, Single Cell Activity Multiplexing, and biofilms
Goal 4: Computational models on the edge of optimality & 4D cell models
Conclusion/Management/Bibliography

Collaborators:
Sallie Chisholm, Martin Polz, Roberto Kolter, Fred Ausubel, Raju Kucherlapati, Steve Lory, Steve Gygi, Mike Laub

May 2007-2012 Proposal
S0: Potential Synergies with other Centers
S1: Improve and Multiplex & Single Cell Genome Sequencing technologies
S2: Development of engineered RNA molecules to control gene expression, sense metabolites, and optimize the properties of metabolic pathways
S3: Proteomics in vitro synthesis, in vivo quantitation, and structural studies
S4: Functional Genomics Analysis of the Soil Bacterium P. aeruginosa and its Interactions
S5: Generation of Metabolic Analysis Models and Evolution
S6: Genomic structure, metagenomics, horizontal gene transfer, and natural diversity of Prochlorococcus and Vibrio
S7: Genome Engineering and the Construction of New Genetic Codes

Collaborators:
Steve Gygi, Susan Leschine

2012-2016 Proposal

2016-2020 Proposal

Related Pages:
Scheduled Project Meetings on Tuesdays at noon. Location: CLSB 521
Other gc-lab grant proposals
DOE Meetings & News
Synthetic Biology Projects
NSF SynBERC Team
This page was updated 6-Dec-2016 by GMC

Related Biofuel & Synthetic Biology Companies